Label-free hyperspectral nonlinear optical microscopy of the biofuel micro-algae Haematococcus Pluvialis
نویسندگان
چکیده
منابع مشابه
Photo Protection of Haematococcus pluvialis Algae by Astaxanthin: Unique Properties of Astaxanthin Deduced by EPR, Optical and Electrochemical Studies
Abstract The antioxidant astaxanthin is known to accumulate in Haematococcus pluvialis algae under unfavorable environmental conditions for normal cell growth. The accumulated astaxanthin functions as a protective agent against oxidative stress damage, and tolerance to excessive reactive oxygen species (ROS) is greater in astaxanthin-rich cells. The detailed mechanisms of protection have remain...
متن کاملProduction of biofuel by using micro algae (Botryococcus braunii)
Microalgae are photosynthetic, heterotrophic organisms that have an extraordinary potential for cultivation as energy crops. Microalgae are potential source of biomass, which may have great biodiversity and consequent variability in their biochemical composition (Satyanarayana et al., 2011). Under difficult agro-climatic conditions and are able to produce a wide range of commercially interestin...
متن کاملLabel-free multimodal nonlinear optical microscopy reveals fundamental insights of skeletal muscle development.
We developed a label-free nonlinear optical (NLO) microscope integrating the stimulated Raman scattering, multi-color two-photon excited fluorescence and second harmonic generation. The system produces multimodal images of protein content, mitochondria distribution and sarcomere structure of fresh muscle samples. With the advanced imaging technique, we studied the mal-development of skeletal mu...
متن کاملLabel-free nonlinear optical microscopy detects early markers for osteogenic differentiation of human stem cells
Tissue engineering by stem cell differentiation is a novel treatment option for bone regeneration. Most approaches for the detection of osteogenic differentiation are invasive or destructive and not compatible with live cell analysis. Here, non-destructive and label-free approaches of Raman spectroscopy, coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) microscop...
متن کاملAntioxidant role of astaxanthin in the green alga Haematococcus pluvialis
The green unicellular alga, Haematococcus pluvialis has two antioxidative mechanisms against environmental oxidative stress: antioxidative enzymes in vegetative cells and the antioxidative ketocarotenoid, astaxanthin, in cyst cells. We added a reagent that generates superoxide anion radicals (O2 ), methyl viologen, to mature and immature cysts of H. pluvialis. Tolerance to methyl viologen was h...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biomedical Optics Express
سال: 2014
ISSN: 2156-7085,2156-7085
DOI: 10.1364/boe.5.003391